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1. | If the QE Pro is out of calibration, the Instrument Calibration screen will appear. |
2. | Click the Calibrate Instruments button to begin the calibration process. The logger moves the QE Pro integrating sphere over the white calibration standard and lowers it until it touches. It will take some preliminary measurements to establish the integrating time based on an 80% saturation level of the total response. These measurements take about 0.30–0.55 seconds for the current light and detector configuration. (Figure 9, below) |
3. | Click Accept for the suggested integration time. The logger will then take 20 measurements of the white standard at the automatic integration time. Figure 9. White Calibration |
4. | After the white measurements are completed, the logger will raise the integrating sphere, position it over the dark standard, and lower it on the dark standard. The shutters on the light sources will close and the dark measurement will acquire 20 measurements, as above. When finished, Click Accept! |
5. | Figure 12 shows the normalization of spectra that will occur with the just-acquired calibration. |
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