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Vsyringe = Volume of the secondary standard extracted via the autosampler or manual syringe.
PFMD Stock Level A (400,000 ng/L)
Using a 10 uL cemented needle syringe, add 2.5 µL of neat PFMD reagent into 12.25 mL of Optima Grade hexane in a headspace vial (mixed hexanes will serve equally well).
Serial Dilutions for working standards
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Prepare serial dilutions of stock level A into separate headspace vials. Cap several BLANK vials in advance before working with PFMD to ensure a tracer free background. Use a cemented needle syringe from SGE Corp. (not a plastic-tip pipettor) to add the specified levels (Table 2) of stock solution A to 20 mL crimp-top magnetic cap headspace vials by injecting through the septum. Use the high precision 1 µL analytical syringe to accurately measure small volumes. Move plunger VERY slowly while drawing and dispensing the solution. You might not be able to see a drop forming at the tip of the needle. Just stay in the vial for a few moments to ensure the entire injection has evaporated into the vial.
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