...
- Place sample in centrifuge tube with 25 mL of 10 % Acetic Acid for at least 1 hour to decarbonate (it helps to place the centrifuge tubes on the agitator in the cold room)
- Please note, that sample with a large amount of carbonate (more than 50%) may require more than 1 treatment of Acetic to reach complete decarbonation.
- Centrifuge for 15 mins at 1500 rpm, decant and wash with 25 mL of nanopure water (3X to remove all Acetic Acid)
...