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17. Adjust the Blue Black gain until the GREEN/BLUE value is 1.

Setting the Line Rate

The rules:

• Exposure intervals for the Red, Green and Blue channels is a function of the light intensity of the LEDs.
• Led intensity for the three channels is a function of the LED spectrum. Cold lights 65K are very blue while warm lights 55k are reddish. Neutral light is preferred where RGB are nearly equal but all leds are variable.  Generally red is the lowest intensity and will require the longest exposure time.  That is why in the above procedure we start with red, but you should always start with lowest intensity channel.
• Intensity is also a function of the F-stop. The smallest F-Top is preferred because it gives the greatest depth of focus but also lowers the intensity.
• Intensity is also a function of how closely coupled the lights are to the core surface but for practical reasons we need to keep a minimum clearance for safe operations.
• Don’t forget to check that all of the lights are evenly illuminated or even on. One led can fail without affecting these other.  Don’t ever look at the leds directly!
• Line rate must be greater (20us) than the red, green and blue exposure rates set in the above procedure.
• The shorter the line rates the faster the image can be scanned.
• If you move the track faster than the scan rate you will see dropped lies in your image.

So you must balance all of these setting to obtain the best scanning rate.  That means the above White balance procedure described above may have to be repeated as you adjust light position, f-stop etcetera.

Note: when you adjust the Line Rate interval it will automatically adjust the speed of the track to a scan speed that will not cause dropped lines.  You can override this value in the Motion Control Setup  …be careful.

LIMS Integration

Sample and Analysis Components

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