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- After removing the supernatant (after the last wash), add ~25 mL of a 1 % Borax solution to the centrifuge tube (Figure 9, Step #8). Borax prevents the sample from flocculating. Too much Borax however will increase flocculation.
- Position the sample with the sonicating probe abut half-way into the sample material. Use the clamp to hold the tube in place (Figure 9, Step#9). The probe should not be touching the sides of the tube or the release of energy from the probe will melt the plastic tube.
- When satisfied with the positioning, flip the "ON" Switch (Figure 10, arrow A). The settings are already set to our needs: Mode = Continuous, Amplitude = 65 (Figure 10). To run as is, press the "Test" button (Figure 10, arrow D), the screen will display 'rdy' when it is ready for use. If Alternatively, if you select Mode = Time (min/sec), the probe will stop automatically after 1.5 minutes. If you need to change a setting, press the "Mode" button (Figure 10, arrow B) and then the "Set" button (Figure 10, arrow C). From these two panels, you have access to the power, time, and displayed units. For more information, reference the Manufacturer Manual located in the side pocket of the dismembrator case (Figure 10).
- Press the "Start" button on the probe power supply (Figure 9, Step #10 and Figure 10, arrow E). If the material does not appear to be circulating throughout the whole tube or the tube is heating up, adjust the probe position and start the dismembration over. Allow the sample to circulate for at least 1 minute, then press the "Stop" button (Figure 10, arrow E). Remove the sample from the clamp. Wipe the probe with a Kimwipe, then clean with isopropyl alcohol and dry with a Kimwipe.
- Leave the sample to settle for about 12 hours on the benchtop (Figure 9, Step #11).
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