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The Epifluorescence microscope in the microbiology laboratory aboard the JOIDES Resolution consists of a Zeiss Axio Imager 2 microscope with transmitted and reflected capabilities, as well as a CoolLED pE-100 single wavelength illumination system for screening fluorescence. The microscope is furthermore/in addition outfitted furthermore outfitted with a Zeiss 506 camera for imaging and the/with ZEN lite blue image capture software.

Cool lights usage

First, switch on the microscope lights and select RL/3200 K:

 

LEDs are switched on and off by pressing the ‘on/off’ button located on the left side of the microscope. At start-up the Light Source pE100 light source will revert to the same settings that were set when it was last powered down. New Light Sources light sources are supplied with the settings as shown below.

Adjust the intensity of the Light Source pE100 light source by pressing the up and down intensity buttons. This is displayed in 1% steps between 20% and 100%. Between 0% and 20% this figure/feature?option? is displayed in 0.2% intensity steps. 


Comment: On the right figure, shouldn't it be "to decrease the light output"? "power" is understandable, just for the sake of consistency (wink)

What does 365 mean?

Camera/Imaging

The microscope has a Zeiss 506 color camera attached and has Zen lite software installed. The sensitivity of the camera is based on a 6 megapixel CCD sensor and has a resolution of 2752 horizontal pixels × 2208 vertical pixels.

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3) On the left side in the tool area, the Set exposure/Live/Continuous and Snap options will become active a few seconds after opening the software. Click Live, be sure to set the microscope light path to the camera (see picture below). A live image will be displayed in the center area of Zen blue.

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7) In the Processing tool, pick the Method Image Export and select JPEG filetype, Export to C:\data\in and Generate xml file, then click Apply. When the user closes the program, the user will be asked if the original .czi file should be saved as well - please confirm and save in the same folder.

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