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Author(s): | D. Houpt (362T) | |
Reviewer(s): |
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Management Approval (Name, Title, Date): | D. Houpt (371T) | |
Audience: | Users of microscopes | |
First Release: | Draft | July 2016 (Exp. 362T) |
Current Version: | 374 | March 2018 |
Revised: | R. Gray, Z. Mateo | |
Domain: | Microscopy | |
System: | Microscope Image Capture |
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AxioPlan Upright Microscope | Stereo Microscope | |
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202-PlanNeoPh2 40x | 1-zoom 0.5x | |
218-ECEpiNeo 50x | 1.25-zoom 0.625x | |
38-PlanNeo 63x | 1.6-zoom 0.8x | |
196-PlanNeoPh3 63x | 2-zoom 1x | |
177-PlanNeo 100x | 2.5-zoom 1.25x | |
198-PlanNeoPh3 100x | 3.2-zoom 1.6x | |
1.25Optovar40x 50x | 4-zoom 2x | |
1.25Optovar50x 62.5x | 5-zoom 2.5x | |
1.25Optovar63x 78.75x | 6.3-zoom 3.15x | |
1.25Optovar100x 125x | 8-zoom 4x | |
1.6Optovar40x 64x | ||
1.6Optovar50x 80x | ||
1.6Optovar63x 100.8x |
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1.6Optovar100x 160x |
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2.0Optovar40x 80x |
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2.0Optovar50x 100x | ||
2.0Optovar63x 126x | ||
2.0Optovar100x 200x |
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Table 2. Combined objective-times-Optovar objectives/objective-times-selector knob, and virtual objectives. The final magnification on the right of each option includes the multiplier for the different adapter tubes.
To keep the list as short as possible, the phase and non-phase objectives were combined on the Axioplan Optovar virtual objectives, but if the user wishes to record that information, each individual objective-Optovar combination can be created.
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In all cases, the expected error is relatively small, but only when all three of the criteria are satisfied.
For the upright microscopes, the expected error in the scale bar is approximately 0.5%.
For the stereo microscopes, the expected error in the scale bar is approximately 0.5-1.0% for those scopes with clickstop magnification knob. For ones without the stop (including "Margarita," the DISCOVERY V8 scope with a malfunctioning rack-and-pinion system), the error is still ≤2% if the user is cautious.
From the camera manufacturer: "Calculated Calibrations are, in reality, mathematical estimates based upon the accuracy of the values input by the user. Because there may be tolerances to consider on any optical element in the system, or simply inaccuracies between "nominal" and actual values, the resulting calibration should be close, but is not a true calibration. For exacting work, it is best to manually calibrate your optical system."
The JRSO agrees.