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The insolubility of PFMCH creates challenges in creating the dilution curve. In order to deal with this problem, a volatile gas dilution scheme was created as follows:
Stock solution is pure PFMCH. Follow the dilution schema scheme in Table 1.
- Using a cemented-needle 10 µL syringe, inject neat PFMCH into calibration level 7.
- Allow the PFMCH aliquot to completely evaporate (~10 minutes).
- Use a gastight syringe to extract 0.5 mL of 7 through the septum, and inject it into vial 6.
- Continue serial dilutions as noted to create all calibration levels as shown in Table 1.
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