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- 20 mL headspace vials (HP 5182-0837) and magnetic metal caps with PTFE/rubber seals
- Note: the PTFE side should face the vial and sample; the rubber side faces outward when the lid is crimped onto the vial
- Manual vial crimper
- 10 mL, 1 mL, and 200 µL syringes
- 0.1–1.0 mL 1uL and 10 uL gas-tight syringes
- Oven gloves and metal tray
- GC septa: 11 mm diameter, usable up to 250°C or 400°C
- GC column: Agilent column (15 m x 0.250 mm x 5 µm)
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Table 2: Serial dilution scheme for PFMD. As of Exp395C, we are only using the Low Level calibration.
Hardware
The GC2 system comprises an HP 6890 gas chromatograph (GC) with a micro-electron capture detector (µECD).
The GC inlet is operated in splitless mode. PFT gas samples obtained using the headspace extraction method may be injected manually after incubation for 30 minutes at 85 deg. C, or can be injected by the Gerstel autosampler (whose incubator oven should be set to 85°C70°C for 600 seconds). The injection port liner assembly is connected to a megabore column (Rt-Alumina BOND/KCl, 50 m, 0.53 mm ID, 10 µm thickness), and then to a µECD detector, which requires both carrier (helium) and makeup gases (nitrogen).
Ensure the syringe installed in the autosampler has the Teflon-tipped plunger (Figure 6).
Figure 6: Different syringes used by the Gerstel Autosampler. The rubber plunger of the syringe shown on top causes significant sample carryover, likely due to tracer penetrating pore spaces within the rubber. It is best to use the syringe with the teflon-tipped plunger shown on bottom.
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