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Figure 1. Metrohm Autotitrator with Workstation (Note: dispenses 0.1 M HCl).
Apparatus, Reagents, & Materials
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LIMS stores several components of each analysis, which are detailed in the chart below.
Analysis | Component | Unit | Description |
ALKALINITY | alkalinity | mM | Ability of a solution to neutralize acid to the equivalence point of carbonate |
pH | NA | Acidity or basicity of a solution (–log[H+]) | |
acid_quantity | µL | Amount of 0.1 M HCl added to the sample during titration | |
correction_factor | — | Correction factor for non-ideal behavior of samples to adjust calibration | |
ALK_QAQC | alkalinity | mM | Ability of a solution to neutralize acid to the equivalence point of carbonate |
pH | NA | Acidity or basicity of a solution (–log[H+]) | |
acid_quantity | µL | Amount of 0.1 M HCl added to the sample during titration | |
correction_factor | — | Correction factor for non-ideal behavior of samples to adjust calibration | |
slope | — | Slope of the calibration equation | |
intercept | — | Intercept value of the calibration equation | |
corr | Rho | Correlation coefficient of the calibration |
Uploading Data to LIMS
When the alkalinity titration finishes, the Alkalinity Calculation screen shows the slope information, correction factors, and final alkalinity value (Figure 14).
Edit outlier Gran factor points, if necessary (see Editing Gran Factor Points). If satisfied with the results, click Done/Save to load the values into LIMS.
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Wear personal protective equipment including close-toed shoes, lab coat, gloves , and safety glasses when working with acids of any strength.
Use a fume hood when making solutions from concentrated acids.
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– | Make sure the probe storage container is filled with 3 M KCl solution and the reservoir is filled with 0.1 M HCl solution. |
– | Ensure no air bubbles are present in the acid dispensing line. Press DOS on the body of the titrator to push acid through the line and remove air bubbles. |
– | Change out the Drierite trap when ~50% of the color turns from blue to pink. |
| – | If you are hearing a bubbling noise, top up your water reservoir. Tap water is fine. Check titration vessel for air bubble and eliminate if present. |
Consumables
Electrode: Metrohm 6.0234.100 or equivalent. |
0.1 M HCL, Fisher CH5009 |
Microvalve buret tip, Metrohm 020683244, CM5129 |