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Liquid Scintillation Counter Quick User Guide
X328T (Sept-Oct 2010)
Chieh Peng
Setup
Computer/Laptop is connected through comp port #2 on the counter
Startup

  1. Insert "Wallac 1414 WinSpecial Ins.Prog "3.5" disc into the front port
  2. Log on to the computer using daq/daq
  3. Open "1414 Winspecial" software on the computer
  4. Turn on the scintillation counter, the power switch is on the back
  5. Winspecial main window status line should show "Loading" > "counter is busy"> "counter online"
  6. Now the counter is ready


Sample Loading

  1. Two types of sample racks, can be used in combination
  2. Locate the barcode on the side of the sample rack. This should be your first rack, so the instrument knows where to "start" the measurement
  3. Barcode should be facing the right hand side, when operator is facing the counter. The first rack should go in the right side of the instrument closer to the back

Power switch
sSample rack direction
barcode
Sample loading direction
To Run Sample/Standard

  1. 5 Sub-windows show up in the Winspecial main window

Easy GLPProtocol for standard reference measurement
Control Live To view Live signal
User BiolabEasy count is default method for unknown sample
Latest Resultshow latest result
Fine TuningSelect spectra to plot or store

  1. Standards used in Perkin Elmer Unquenched LSC-std, Loading order is 3H, 14C, and blank
  2. Out standard have the following strength. This information is entered in Easy GLP

3H196600 2001/7/01 half-life is 12.35 yrs
14C101000 2001/7/01 half-life is 5730 yrs

  1. Instrument will calculate base on the half-life and give you the current strength in comparison to the measurement result.
  2. Easy counter cam measure 3H, 14C, 125I, 32P, 35S, and 45Ca. We only carry the first 2 standards.
  3. Easy counter test value, please refer to software manual P67.


Safety

  1. Store all the standards in the grey plastic boxes when they are not being used.
  2. Wash hands with soap after touching the standards and other potential contaminated samples.
  3. Always follow RadVan user protocol.
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