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b. Take note of how thick the slides are and write it on the sample tray. The samples will need to be 30 microns on top of the of the slide thickness.
3. Place the slides on the heat pad as well.
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- Mix one full pump of EpoTek 301 part A and part B into a weighing dish.
- The ratio is 3 to 1. The pumps are preset to this.
- Make sure pump is primed first or there will not be the right ratio.
- Stir the resin slowly with wooden stick until there are no trails of the two mixing (Fig. 1) and go slow to avoid bubbles.
Figure 1
Make sure no wooden splinters fall wood particles fall into the resin; pick them out if they do.
The resin should stay good for about 45 minutes. If it starts to get gooeyget too thick, place place on the hot plate for a few seconds and it will become runny become thin again for a very short time.
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- Once the samples are completely dry clean the surface off with a kimwipe kim-wipe and isopropyl alcohol.
- Place the billet on a Teflon puck.
- With the wooden stick slowly draw an X with the resin on the sample (Fig. 2).
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